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Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil
Author(s) -
Koch MO,
Weiss RR,
Cruz AA,
Soccol VT,
Gonçalves KA,
Bertol MAF,
Beltrame OC,
Dittrich RL
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12718
Subject(s) - toxoplasma gondii , semen , biology , parasite hosting , protozoa , polymerase chain reaction , virology , microbiology and biotechnology , toxoplasmosis , in vitro , vero cell , antibody , immunology , virus , gene , genetics , world wide web , computer science
Contents The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa ( TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii .