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Laminin‐111 Inhibits Bovine Fertilization but Improves Embryonic Development in vitro , and Receptor Integrin‐ β 1 is Involved in Sperm–Oocyte Binding
Author(s) -
Lin F,
Huang Cj,
Liu Cs,
Guo Ll,
Liu G,
Liu Hj
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12716
Subject(s) - laminin , oocyte , blastocyst , biology , sperm , andrology , human fertilization , embryogenesis , integrin , microbiology and biotechnology , embryonic stem cell , embryo , receptor , anatomy , extracellular matrix , genetics , medicine , gene
Contents This study detected the distribution of laminin during embryonic formation by immunofluorescence. To determine the possible function of laminin on developmental ability of in vitro fertilized embryos, the presumptive zygotes were divided and transferred to CR 1aa medium supplemented with different concentrations (0 μg/ml, 5 μg/ml, 10 μg/ml and 20 μg/ml) of laminin. To explore the association with sperm–oocyte fusion, oocytes and/or sperm were pre‐incubated with laminin or anti‐ β 1 antibody before insemination. Laminin was absent in mature oocytes and could be detected first at the 8‐cell stage and then displayed an increasing tendency. Adding 10 μg/ml laminin to the culture medium improved embryonic development including cleavage rate, blastocyst rate, total cell numbers in the blastocyst and cell numbers in the inner cell mass. Laminin inhibited sperm–oocyte fusion when incubated with oocytes and/or sperm before in vitro fertilization, and only integrin‐ β 1 of sperm was involved in sperm–oocyte binding. Inhibition may be caused by blocking β 1, but why laminin inhibits fertilization is still unknown. The results suggest that laminin plays an important role during embryonic formation and has a negative function in sperm–oocyte fusion, but improves embryonic development. However, only integrin‐ β 1 is involved in sperm–oocyte binding.