Premium
Effects of Interferon‐Tau and Steroids on Cytochrome P450 Activity in Bovine Endometrial Epithelial Cells
Author(s) -
Gilfeather CL,
Lemley CO
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12695
Subject(s) - cytochrome p450 , endocrinology , medicine , monooxygenase , oxytocin , interferon , chemistry , pharmacology , biology , metabolism , immunology
Contents The objective of the current study was to examine cyclooxygenase ( COX ), cytochrome P450 1A ( CYP 1A) and 2C ( CYP 2C) activity in bovine endometrial cell cultures following exposure to oxytocin ( OT ), interferon‐ τ ( IFN ), estradiol (E2) and/or progesterone (P4). Bovine endometrial epithelial cells were treated with OT , IFN , a combination of OT + IFN or control ( CON ) media for 24 h. For the second experiment, cells were treated with E2, P4, a combination of E2 + P4 or CON media for 24 h. Treatments were performed in triplicate, and the experiment was repeated four times (n = 12 per treatment). Treatment with OT alone increased (p < 0.01) activity of COX compared with CON ; however, OT alone did not alter activity of CYP 1A (p = 0.55) or CYP 2C (p = 0.46) compared with CON . Activity of CYP 1A and CYP 2C was decreased in cells exposed to IFN (p < 0.01) or OT + IFN (p < 0.01) compared with CON . Treatment with E2 alone did not alter activity of CYP 1A (p = 0.64) or CYP 2C (p = 0.06) compared with CON . Activity of CYP 1A and CYP 2C was decreased (p < 0.01) in P4 vs CON . In summary, IFN exposure, irrespective of OT treatment, decreased the activity of CYP 1A and CYP 2C. Activity of CYP 1A was decreased following P4 treatment alone, while that of CYP 2C was decreased following both P4 and E2 + P4 treatment. The mixed function monooxygenase enzymes, CYP 1A and CYP 2C, have been implicated in synthesizing embryotoxic compounds; therefore, downregulation in the endometrium may be necessary during maternal recognition of pregnancy.