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l ‐carnitine Mediated Reduction in Oxidative Stress and Alteration in Transcript Level of Antioxidant Enzymes in Sheep Embryos Produced In Vitro
Author(s) -
Mishra A,
Reddy IJ,
Gupta PSP,
Mondal S
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12682
Subject(s) - carnitine , embryo , blastocyst , andrology , antioxidant , oxidative stress , glutathione , chemistry , incubation , biology , intracellular , in vitro , enzyme , embryogenesis , endocrinology , medicine , biochemistry , microbiology and biotechnology
Contents The objective of this study was to find out the effect of l ‐carnitine on oocyte maturation and subsequent embryo development, with l ‐carnitine‐mediated alteration if any in transcript level of antioxidant enzymes (GPx, Cu/Zn‐ SOD ( SOD 1) and Mn‐ SOD ( SOD 2) in oocytes and developing sheep embryos produced in vitro . Different concentrations of l ‐carnitine (0 m m , 2.5 m m , 5 m m , 7.5 m m and 10 m m ) were used in maturation medium. Oocytes matured with 10 m m l ‐carnitine showed significantly (p < 0.05) higher cleavage (66.80% vs 39.66, 41.76, 44.64, 64.31%), morula (48.50% vs 20.88, 26.01, 26.99, 44.72%) and blastocyst (33.22% vs 7.66, 9.19, 10.71, 28.57%) percentage as compared to lower concentrations (0 m m , 2.5 m m , 5 m m and 7.5 m m ). Cleavage percentage between 10 m m and 7.5 m m l ‐carnitine were not significantly different. Maturation rate was not influenced by supplementation of any experimental concentration of l ‐carnitine. There was a significant (p < 0.05) decrease in intracellular ROS and increase in intracellular GSH in 10 m m l ‐carnitine‐treated oocytes and embryos than control group. Antioxidant effect of l ‐carnitine was proved by culturing oocytes and embryos with H 2 O 2 in the presence of l ‐carnitine which could be able to protect oocytes and embryos from H 2 O 2 ‐induced oxidative damage. l ‐carnitine supplementation significantly (p < 0.05) upregulated the expression of GPx and downregulated the expression of SOD 2 genes, whereas the expression pattern of SOD 1 and GAPDH (housekeeping gene) genes was unaffected in oocytes and embryos. It was concluded from the study that l ‐carnitine supplementation during in vitro maturation reduces oxidative stress‐induced embryo toxicity by decreasing intracellular ROS and increasing intracellular GSH that in turn improved developmental potential of oocytes and embryos and alters transcript level of antioxidant enzymes.