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Can the Genetic Origin Affect Rabbit Seminal Plasma Protein Profile along the Year?
Author(s) -
CasaresCrespo L,
Talaván AM,
ViudesdeCastro MP
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12680
Subject(s) - polyacrylamide gel electrophoresis , biology , blood proteins , microbiology and biotechnology , gel electrophoresis , protein subunit , two dimensional gel electrophoresis , endocrinology , gene , genetics , proteomics , biochemistry , enzyme
Contents The study was designed to evaluate the influence of genetic origin on rabbit seminal plasma protein profile variation along the year. Seminal plasma of rabbits from line A (maternal line) and R (paternal line) collected during a natural year was subjected to polyacrylamide gel electrophoresis ( SDS ‐ PAGE ). The electrophoretic profile of rabbit seminal plasma resulted in multiple protein bands of different intensity ranging from 9 to 240 kDa. Results showed that seven protein bands were significantly different between genetic lines, and among these, three protein bands were significantly different between seasons. The differentially expressed proteins were identified by MALDI ‐ TOF / TOF or LC ‐ MS / MS analysis and were the following ones: FAM 115E‐like (220, 113 and 59 kDa), ectonucleoside triphosphate diphosphohydrolase 3 isoform X2 (72 kDa), annexin A5 (32 kDa), lipocalin allergen Ory c 4 precursor (19 kDa), and haemoglobin subunit zetalike (13 kDa) between genetic lines and FAM 115E‐like (113 kDa), haemoglobin subunit zetalike (13 kDa) and β ‐nerve growth factor (12 kDa) between seasons. These results indicate that proteins from rabbit seminal plasma are under both seasonal control and genetic control. Furthermore, the differential presence of these proteins could be one of the causes explaining the differences observed in fertility and seminal parameters between these two lines in earlier studies.