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Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle
Author(s) -
Ilha GF,
Rovani MT,
Gasperin BG,
Ferreira R,
Macedo MP,
Neto OA,
Duggavathi R,
Bordig V,
Gonçalves PBD
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12662
Subject(s) - follicular phase , anti müllerian hormone , medicine , endocrinology , follicular fluid , biology , follicle , hormone , messenger rna , andrology , oocyte , embryo , gene , microbiology and biotechnology , biochemistry
Contents The anti‐Müllerian hormone ( AMH ) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor ( AMHR 2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant ( DF ) than subordinate follicles ( SF ) at the expected time (Day 3) and after (Day 4) deviation (p < 0.05). In experiment 1, AMH mRNA levels declined in both DF and SF near the expected time and after deviation when compared to before deviation. There was no difference in AMHR 2 mRNA levels before and during follicular deviation (p > 0.05), but they tended to be greater in DF s than SF s (p < 0.1) after deviation. Experiment 2 showed that AMH and AMHR 2 mRNA in granulosa cells and AMH protein abundance in follicular fluid were similar (p > 0.05) between both co‐dominant follicles collected from the FSH ‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DF s and SF s during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR 2 mRNA expression in granulosa cells of co‐dominant follicles.