Premium
Involvement of Protein cAMP ‐dependent Kinase, Phospholipase A 2 and Phospholipase C in Sperm Acrosome Reaction of Chinchilla lanigera
Author(s) -
GramajoBühler MC,
Zelarayán L,
SánchezToranzo G
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12659
Subject(s) - acrosome reaction , capacitation , sperm , protein kinase a , biology , acrosome , phospholipase c , protein kinase c , phospholipase a2 , andrology , human fertilization , in vivo , arachidonic acid , medicine , endocrinology , microbiology and biotechnology , signal transduction , kinase , biochemistry , anatomy , enzyme , genetics
Contents The mechanisms involved in fertilization are the centre of attention in order to determine the conditions required to reproduce in vitro the events that take place in vivo , with special interest in endangered species. Previous data from mouse sperm, where acrosome reaction ( AR ) occurs more often in the interstitium of the cumulus oophorus, contribute to strengthen the use of progesterone as a physiological inducer of this process. We studied the participation of protein kinase A ( PKA ), phospholipases A 2 and C ( PLA 2 , PLC ) in the AR induced by progesterone from Chinchilla epididymal spermatozoa. The addition of db‐ cAMP to the incubation medium caused an increase of 58% in the AR , while the use of H89 (30 μ m ), a PKA inhibitor, reflected a decrease of 40% in the percentage of reacted gametes. The assays conducted with arachidonic acid showed a maximum increase of 23% in the AR . When gametes were pre‐incubated with PLA 2 inhibitors, a dose‐dependent inhibitory effect was observed. The addition of phorbol12‐myristate13‐acetate (10 μ m ) revealed higher percentages of AR induction (60%). When PLC was inhibited with neomycin and U73122, a dose‐dependent decrease in AR percentages was observed. Combined inhibition of PKA , PLA 2 and PLC , AR values similar to control were obtained. This work shows evidence, for the first time in Chinchilla, that progesterone activates the AC / cAMP / PKA system as well as sperm phospholipases and that these signalling pathways participate jointly and cooperatively in AR . These results contribute to the understanding of the complex regulation that is triggered in sperm after the effect of progesterone.