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Prolactin, Androstenedione and IGF 1 Serum Concentrations During Induced Follicular Growth by e CG Administration in the Bitch
Author(s) -
Stornelli MC,
García Mitacek MC,
Praderio RG,
Nuñez Favre R,
Sota RL,
Stornelli MA
Publication year - 2016
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12656
Subject(s) - estrous cycle , prolactin , endocrinology , follicular phase , medicine , androstenedione , hormone , chemistry , biology , androgen
Contents The oestrus cycle in the domestic bitch, a monoestrous species, differs considerably from that of other veterinary domestic animals species. In the bitch the combined use of e CG and h CG is effective to induce oestrus predictably and safely (Stornelli et al., Theriogenology, 78, 2012 and 1056). Although several studies were done to describe the hormonal changes during the canine oestrus cycle, to our knowledge none was done to describe the hormonal changes during induced follicular growth after the administration of e CG . The aim of this work was to study prolactin ( PRL ), insulin‐like growth factor ( IGF 1) and androstenedione ( ANDR ) serum concentrations during follicular growth induced by a single dose of e CG administered to late anoestrous bitches. PRL and ANDR concentrations were lower before than after e CG TRT (before e CG vs pro‐oestrus, oestrus and dioestrus; 4.3 ± 1.8 ng/ml vs 6.5 ± 1.6 ng/ml, p < 0.05; 0.08 ± 0.2 ng/ml vs 0.42 ± 0.16 ng/ml, p < 0.05). Conversely, IGF1 concentrations were similar before and after e CG TRT (286.0 ng/ml ±32.2, p > 0.53). Additionally, PRL concentrations were similar before oestrus compared to during oestrus and dioestrus (6.9 ± 1.7 ng/ml, p > 0.19). Furthermore, IGF 1 concentrations were higher before and during oestrus compared to first day of dioestrus (286.1 ± 29.8vs 200.4 ± 29.2 ng/ml, p < 0.01). On the contrary, ANDR concentrations were lower before and during oestrus compared to first day of diestrum (0.35 ± 0.17 ng/ml and 0.38 ± 0.15 vs 0.68 ± 0.17 ng/ml, p < 0.05). These results show that treatment with a single injection of 50 IU/kg of eCG in late anoestrous bitches successfully induced changes in follicular growth which were paralleled with changes in PRL , IGF 1 and ANDR serum concentration similar to those occurring during a normally occurring oestrous cycle. In addition, our results suggest that IGF 1 in the bitch could play an important role in ovarian folliculogenesis.