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Expression of m RNA Encoding the LH Receptor ( LHR ) and LHR Binding Protein in Granulosa Cells from N elore ( B os indicus ) Heifers Around Follicle Deviation
Author(s) -
Ereno RL,
Loureiro B,
Castilho ACS,
Machado MF,
Pegorer MF,
Satrapa RA,
Nogueira MFG,
Buratini J,
Barros CM
Publication year - 2015
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12614
Subject(s) - ovulation , messenger rna , follicle , biology , andrology , endocrinology , medicine , receptor , gene , hormone , biochemistry
Contents The time at which follicles acquire LHR in bovine granulosa cells is the subject of some controversy among researchers. The main objective of the present study was to assess the mRNA expression of LHR and LRBP ( mRNA protein binding), a post‐transcriptional suppressor of LHR mRNA expression, in granulosa cells from the two largest follicles around the expected time of follicle deviation in Nelore heifers. First, the interval between ovulation and follicle deviation in 20 Nelore heifers was determined (2.3 ± 0.2 days after ovulation). Ovulation was hormonally synchronized, and then, heifers were slaughtered on days 2, 2.5 and 3 after ovulation (before, during and after, respectively, the expected time of follicle deviation), and granulosa cells from the two largest follicles were collected. The m RNA abundance of an LHR fragment common to all isoforms (total LHR ) and LRBP was assessed by real‐time RT ‐ PCR , and LHR alternative transcripts were assessed by semiquantitative RT ‐ PCR followed by electrophoresis. LHR m RNA expression was not detected before the expected time of deviation. Total LHR m RNA abundance was greater in the largest follicle and increased from day 2.5 to 3. In contrast, LRBP mRNA was detected starting on day 2 and was more expressed in the second largest follicle on days 2.5 and 3. The present data suggest that the expression of LHR mRNA in bovine granulosa cells is established after follicle deviation and that the lower abundance of LRBP m RNA after the expected time of deviation may contribute to greater expression of LHR in the bovine dominant follicle.