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Rotation of Boar Semen Doses During Storage Affects Sperm Quality
Author(s) -
Schulze M,
Rüdiger K,
Waberski D
Publication year - 2015
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12532
Subject(s) - extender , semen , sperm , boar , sperm quality , andrology , sperm motility , motility , semen extender , semen quality , biology , membrane integrity , chemistry , medicine , membrane , biochemistry , microbiology and biotechnology , organic chemistry , polyurethane
Contents It is common practice to rotate boar semen doses during storage for prevention of sperm sedimentation. In this study, the effect of rotation of boar semen doses during storage on sperm quality was investigated. Manual turning twice daily and automatic rotation five times per hour resulted in the following effects: alkalinization of the BTS‐extender, loss of membrane integrity at day 3, and loss of motility and changes in sperm kinematics during a thermoresistance test at day 5. Using a pH ‐stabilized variant of BTS extender, sperm motility and velocity decreased in continuously rotated samples, whereas membrane integrity and mitochondrial activity remain unaffected. It is concluded that rotation of semen samples adversely affects sperm quality and, therefore, should no longer be recommended for AI practice.