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Interactions Among Different Devices and Electrical Stimulus on the Electroejaculation of Captive Agoutis ( Dasyprocta leporina )
Author(s) -
Castelo TS,
Souza ALP,
Lima GL,
Peixoto GCX,
Campos LB,
Oliveira MF,
Silva AR
Publication year - 2015
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12517
Subject(s) - electroejaculation , semen , sperm retrieval , semen collection , square wave , tylosin , semen analysis , stimulation , intracytoplasmic sperm injection , medicine , biology , andrology , sperm motility , artificial insemination , infertility , physics , pregnancy , microbiology and biotechnology , genetics , antibiotics , quantum mechanics , voltage
Contents The interactions among different electroejaculation devices associated with serial or continuous stimuli were investigated to improve the efficiency of the electroejaculation for semen collection in agoutis. Ten sexually matured male Dasyprocta leporina were restrained by the intramuscular administration of xylazine–ketamine association. Each individual was randomly subjected to four electroejaculation protocols, by combining two devices (one presenting longitudinal electrodes emitting square waves and other presenting ring electrodes emitting sine waves) and two electrical stimuli protocols (serial or continuous). A total of 40 attempts for electroejaculation were conducted in agoutis, being 10 per treatment. The most efficient treatment in providing ejaculates containing sperm (p < 0.05) was that using and electroejaculator connected to a probe with ring electrodes and associated with serial stimuli (4/7; 57%). In spite of semen parameters obtained by sine waves were adequate for using the samples for assisted reproduction, higher values for sperm motility and functional membrane integrity were obtained in the use of the square wave, independently of the electric stimulation protocol used (p < 0.05). In conclusion, we verified that the use of a device presenting a probe with ring electrodes and emitting sine waves, associated with a serial stimuli protocol, improves the efficiency for semen obtaining by electroejaculation in adults D. leporina .