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Effect of the Interaction Between Cryoprotectant Concentration and Cryopreservation Method on Frozen/Thawed Chicken Sperm Variables
Author(s) -
Abouelezz FMK,
Castaño C,
ToledanoDíaz A,
Esteso MC,
LópezSebastián A,
Campo JL,
SantiagoMoreno J
Publication year - 2015
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12464
Subject(s) - cryoprotectant , straw , cryopreservation , pellet , sperm , semen , sperm motility , chemistry , glycerol , andrology , zoology , acrosome , biology , botany , anatomy , biochemistry , embryo , medicine , ecology , fishery , inorganic chemistry
Contents This work examines the effect of the interaction between different concentrations of two cryoprotectants – glycerol ( GLY ) and dimethylacetamide ( DMA ) – and two methods of cryopreservation – pellets produced by plunging into liquid nitrogen and gradual in‐straw freezing – on frozen/thawed chicken sperm variables. Sperm was cryopreserved using: (i) 6% DMA , following the in‐straw and the pellet methods (ii) 11% GLY , following the in‐straw and the pellet methods; and (iii) 8% GLY in the in‐straw method and 3% DMA in the pellet method (i.e. reduced cryoprotectant concentrations). When 6% DMA was used as the cryoprotectant, no differences were seen between the in‐straw and pellet methods in terms of frozen/thawed sperm variables or fertility (10.8% and 12.8%, respectively). The viability and motility variables of the frozen/thawed sperm produced using the in‐straw method with 11% GLY were higher (p < 0.05) than those recorded for the sperm preserved using the same cryoprotectant and concentration in the pellet method. However, fertility was extremely low in both groups (2.1% and 4.2% for the in‐straw and pellet methods, respectively). Finally, the use of 8% GLY in the in‐straw method returned higher sperm viability, intact acrosome and motility values than the use of 3% DMA in the pellet method (p < 0.01). No differences were seen, however, in the fertility results obtained (28.8% and 25.0%, respectively). These results suggest that cryoprotectant concentrations can be reduced and still provide acceptable fertility rates.