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Effects of Polychlorinated Biphenyls 28, 30 and 118 on Bovine Spermatozoa In Vitro
Author(s) -
Yurdakok B,
Tekin K,
Daskin A,
Filazi A
Publication year - 2015
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12447
Subject(s) - motility , semen , andrology , endocrine system , semen quality , sperm motility , acrosome , in vitro , acrosome reaction , biology , chemistry , sperm , endocrinology , hormone , medicine , biochemistry , genetics
Contents Decline of semen quality due to endocrine‐disrupting chemicals is of concern globally. Among endocrine disrupting chemicals (EDC), Polychlorinated biphenyls ( PCB ) are associated with reduced semen quality in various epidemiological studies. In this study, we evaluated the direct effects of selected PCB s (28, 30 and 118) on fresh spermatozoa of Simmetial bulls aged 2–4 years were evaluated in vitro by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and computer assisted semen analysis (CASA) ( SCA ; Microptics) analysis. IC 50 values were found as 8.45, 5.45 and 9.55 ng/ml for PCB 28, 30 and 118, respectively. Total motility, progressive motility and viability decreased dependent on dose and duration of exposure (0, 2, 4 h). Total motility at IC 50 doses decreased the most in PCB 28 (72.24%) followed by 30 (60.75%) and 118 (64.77%) at 2nd hour following exposure. Motility results were found to be in accordance with the vitality and morphology data where total abnormalities (especially reacted acrosome rate) were found to have increased.