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Expression of Perilipin 2 ( PLIN 2) in Porcine Oocytes During Maturation
Author(s) -
Zhang RN,
Fu XW,
Jia BY,
Liu C,
Cheng KR,
Zhu SE
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12386
Subject(s) - perilipin , biology , microbiology and biotechnology , andrology , biochemistry , medicine , lipolysis , adipose tissue
Contents Perilipins have been reported to limit the interaction of lipases with neutral lipids within the droplets, thereby regulating neutral lipid accumulation and utilization. This study aimed to identify the location and expression of PLIN 1 and PLIN 2 in porcine oocytes during maturation. Quantitative real‐time polymerase chain reaction (q RT ‐ PCR ), immunostaining and Western blot methods were used to characterize the expression and distribution patterns of PLIN 1 and PLIN 2 in porcine oocytes. The results showed that PLIN 1 was not detectable in porcine oocytes. PLIN 2 and BODIPY 493/503‐detected neutral lipid droplets appeared identical distribution patterns and extensive colocalization in both GV and MII porcine oocytes. PLIN 2 protein expression was higher in GV oocytes than that in MII oocytes (p < 0.05), although PLIN 2 m RNA expression was similar in both groups. These findings suggested that PLIN 2 was a major lipid droplet‐associated protein in porcine oocytes.