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MALDI ‐ MS Lipid Profiles of Oocytes Recovered by Ovum Pickup from B os indicus and 1/2 indicus × taurus with High vs Low Oocyte Yields
Author(s) -
SilvaSantos KC,
Ferreira CR,
Santos GMG,
Eberlin MN,
Siloto LS,
Rosa CO,
Marcantonio TN,
Seneda MM
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12352
Subject(s) - antral follicle , oocyte , biology , andrology , embryo , zoology , endocrinology , ovary , genetics , medicine
Contents The aim of the present study was to compare the lipid profile in oocytes of indicus and 1/2 indicus × taurus cows with high and low antral follicle count ( AFC )/oocyte yields. After an OPU procedure (D0), antral follicles ≥3 mm were counted by ultrasonography (D4, 19, 34, 49, 64), and cows were assigned to groups with either high AFC (≥30 follicles; indicus , NH group; 1/2 indicus × taurus , AH group) or low AFC (≤15 antral follicles; indicus , NL group; 1/2 indicus × taurus , AL group). The lipid profiles of the oocytes were determined by MALDI ‐ MS . For GI , GII and GIII oocytes, the indicus samples tend to cluster separately from the 1/2 indicus × taurus samples. The lipid species [ PC ( P ‐38:5) + H ] + and/or [ PC ( P ‐36:2) + N a] + , [ PC (38:2) + H ] + , [ PC (38:5) + N a] + and [ TAG (60:8) + NH 4 ] + were more abundant in indicus ( NH and NL groups) than 1/2 indicus × taurus . The higher lipid content in the indicus oocytes likely reflects differences in the rate of lipid metabolism and may contribute to oocyte competence and embryo development.