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An Immunohistochemical Observations on the Oviduct of the Goat
Author(s) -
Marettová E,
Maretta M
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12349
Subject(s) - oviduct , desmin , epithelium , cytokeratin , elastin , biology , microbiology and biotechnology , anatomy , actin , chemistry , immunohistochemistry , vimentin , endocrinology , immunology , genetics
Contents The oviduct has an important role in regulating transport of gametes and fertilization. The main role in these functions has a smooth muscle cells and ciliated epithelium lining the oviduct. All functions are under the influence of hormonal and nervous system. The objective of this study was immunohistochemically to examine the following structures: lining epithelium, smooth muscle cells, elastic fibres and nerve fibres. For this purpose, the following antibodies were used: cytokeratin 18, S‐100 protein, acetylated α ‐tubulin, smooth muscle actin, desmin and elastin. Ciliary and secretory cells of the lining epithelium were positive for cytokeratin 18 and S‐100 protein. Cilia and the basal body‐associated structures of ciliary cells were positive to acetylated α ‐tubulin. Smooth muscle cells ( SMC ) in mucosa and of the muscular layer were positive for α ‐smooth muscle actin ( SMA ) and desmin. High density of nerve fibres positively reacted to acetylated α ‐tubulin and S100 protein was present in the mucosa, muscular layer and serosa. Elastic fibres positive for elastin form a dense network at the base of the mucosal folds and in the muscle layer. A dense network of these fibres is accompanying the blood vessels. It is supposed that together with smooth muscle cells they are involved in the transport of ovum and in blood flow regulation.