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Improvement in In Vitro Fertilization Rate, Decrease in Reactive Oxygen Species and Spermatozoa Death Incidence in Rams by Dietary Fish Oil
Author(s) -
Matini Behzad A,
Ebrahimi B,
Alizadeh AR,
Esmaeili V,
Dalman A,
Rashki L,
Shahverdi AH
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12328
Subject(s) - human fertilization , sperm , biology , zoology , andrology , semen , botany , anatomy , medicine
Contents Our aim was to evaluate the effects of fish oil feeding on sperm classical parameters, level of reactive oxygen spices ( ROS ), spermatozoa death incidence and in vitro fertilization ( IVF ) rate in rams. We randomly assigned nine rams, into two experimental groups (isoenergetic and isonitrogenous rations with constant level of vitamin E supplement): control ( CTR ; n = 5) and fish oil ( FO ; n = 4, 35 g/day/ram). Diets were fed for 70 days during the physiological breeding season. After a 21‐day dietary adaptation period, semen was collected weekly from each ram by an artificial vagina. Sperm classical parameters were determined by the computer‐assisted sperm analyzer system ( CASA ), and it was prepared for IVF process by swim‐up technique. These evaluations were performed during the first and last weeks of sampling. Intracellular ROS level and spermatozoa death incidence were detected by flow cytometry on a weekly basis after adaptation. Data were analysed with SPSS 15. The volume, concentration (3.6 and 2.7 × 10 9 /ml) and sperm progressive motility (60 and 48%) were significantly improved in the FO group compared with the CTR (p < 0.05). A comparison of two‐cell stage embryos following IVF in the two groups showed a significantly higher fertilization rate in the FO group (56%) compared with the CTR (49%). Superoxide anion (O 2 − ) rate was significantly lower (p < 0.05) at the third week of sampling in the FO . Although the H 2 O 2 rate was numerically lower in the FO group compared with the CTR , this difference was not significant. In addition, apoptosis showed a significant difference in the third week of sampling (15 and 30% for FO and CTR , respectively; p < 0.05). Overall, adding fish oil to the ram diet not only improved sperm quality and IVF results, it also could reduce oxygen‐free radicals and the incidence of spermatozoa death.