z-logo
Premium
IFN ‐ τ Acts in a Dose‐Dependent Manner on Prostaglandin Production by Buffalo Endometrial Stromal Cells Cultured in vitro
Author(s) -
Chethan SG,
Singh SK,
gsiej J,
Rakesh HB,
Singh RP,
Kumar N,
Agarwal SK
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12287
Subject(s) - stromal cell , prostaglandin , collagenase , endocrinology , endometrium , medicine , prostaglandin e2 , in vitro , andrology , biology , chemistry , microbiology and biotechnology , enzyme , biochemistry
Contents Interferon‐ τ ( IFN ‐ τ ) has been recognized as the primary embryonic signal responsible for maternal recognition of pregnancy. Uterine endometrium produces both prostaglandin F 2 α ( PGF 2 α ) and prostaglandin E 2 ( PGE 2 ). PGF 2 α is responsible for the luteolysis; however, PGE 2 favours establishment of pregnancy by its luteoprotective action. In this study, the dose‐response effect of recombinant bovine IFN ‐ τ (rb IFN ‐ τ ) on prostaglandin ( PG ) production by buffalo endometrial stromal cells cultured in vitro was studied. Buffalo endometrial stromal cells were isolated by double enzymatic digestion, initially with trypsin III followed by a cocktail of trypsin III , collagenase type II and DN ase I and subsequently cultured till confluence. Further, cells were treated with different doses of rb IFN ‐ τ (0.001, 0.01, 0.1, 1.0 and 10 μg/ml) and keeping a separate set of control. Culture supernatant was collected after 6, 12 and 24 h of treatment. PG levels in the culture supernatant were measured by enzyme immune assay ( EIA ) and total cellular protein estimated by Bradford method. Results indicated that buffalo endometrial stromal cells following rb IFN ‐ τ treatment enhanced the secretion of both PGE 2 and PGF 2 α , and also its ratio in a strict dose‐dependent manner with a significant increase (p < 0.01) in PGE 2 production at 1 μg/ml dose of rb IFN ‐ τ and maximal stimulation for both PG was observed at 10 μg/ml. Further, both PG production and its ratio were increased significantly (p < 0.01) in a time‐dependent fashion in all the groups at 6, 12 and 24 h post‐treatment with highest level achieved at 24 h as compared with control. Absolute levels of PGE 2 remained higher than PGF 2 α indicating PGE 2 as the major PG produced by endometrial stromal cells. The dose‐dependent response of rb IFN ‐ τ signifies the importance of optimum concentration of IFN ‐ τ for the embryonic development especially during the critical period to establish successful pregnancy.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here