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Expression of Adiponectin and its Receptors in the Porcine Hypothalamus During the Oestrous Cycle
Author(s) -
Kaminski T,
Smolinska N,
Maleszka A,
Kiezun M,
Dobrzyn K,
Czerwinska J,
Szeszko K,
Nitkiewicz A
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12282
Subject(s) - adiponectin , medicine , endocrinology , adiponectin receptor 1 , estrous cycle , hypothalamus , receptor , biology , luteal phase , gene expression , hormone , gene , obesity , biochemistry , insulin resistance
Contents Adiponectin is a hormonal link between obesity and reproduction, and its actions are mediated by two types of receptors: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). This study compares the expression levels of adiponectin and adiponectin receptor m RNA s and proteins in selected areas of the porcine hypothalamus responsible for G n RH production and secretion: the mediobasal hypothalamus ( MBH ), pre‐optic area ( POA ) and stalk median eminence ( SME ). The tissue samples were harvested on days 2–3, 10–12, 14–16 and 17–19 of the oestrous cycle. Adiponectin m RNA expression in MBH was significantly lower on days 14–16, whereas in SME , the most pronounced gene expression was found on days 2–3 of the cycle (p < 0.05). Adiponectin protein in MBH was most abundant on days 17–19 and in POA on days 2–3 (p < 0.05). Adiponectin protein expression in SME was at similar level throughout the most of the cycle with a statistically significant drop (p < 0.05) on days 14–16. AdipoR1 gene expression in POA was potentiated on days 2–3 and 10–12 of the oestrous cycle (p < 0.05). In SME , the highest AdipoR1 m RNA expression was noted on days 2–3 (p < 0.05). The concentrations of the AdipoR1 protein in POA were similar throughout the luteal phase (days 2–14 of the cycle), and they decreased on days 17–19 (p < 0.05). In SME , AdipoR1 protein expression peak occurred on days 2–3 (p < 0.05). The expression patterns of the AdipoR2 gene in MBH , POA and SME revealed the highest m RNA levels on days 2–3 of the cycle (p < 0.05). The highest content of AdipoR2 protein in MBH was reported on days 2–3 (p < 0.05), while in POA on days 17–19 and in SME on days 10–12 and 14–16 (p < 0.05). This study demonstrated that adiponectin and adiponectin receptor m RNA s and proteins are present in the porcine hypothalamus and that their expression levels are determined by the pig's endocrine status related to the oestrous cycle.