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Use of Eggs Derived From the Interspecific Charr Hybrids to Induce Androgenetic Development of the Brook Charr ( Salvelinus fontinalis Mitchill 1814)
Author(s) -
Michalik O,
Dobosz S,
Wójcik I,
Zalewski T,
Ocalewicz K
Publication year - 2014
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12248
Subject(s) - salvelinus , biology , fontinalis , hybrid , ploidy , zoology , offspring , sperm , trout , salmonidae , ecology , genetics , fishery , salmo , botany , gene , fish <actinopterygii> , pregnancy
Contents Although, brook charr ( S alvelinus fontinalis M itchill 1814) and A rctic charr ( S alvelinus alpinu s L innaeus 1758) are able to cross and give fertile offspring, their androgenetic nucleocytoplasmic hybrids are not viable. To overcome incompatibility between the egg cytoplasm of one charr species and the sperm nucleus of another charr species, application of F 1 interspecific hybrids as egg donors for the purpose of androgenesis has been proposed. Here, androgenetic development of the brook charr was successfully induced in the brook charr eggs and the eggs derived from the reciprocal brook charr × A rctic charr F 1 hybrids. A working androgenesis protocol included inactivation of the maternal nuclear DNA achieved by irradiation of the eggs with 420 G y of X ‐rays, insemination of such treated eggs with the haploid sperm cells and exposition of the haploid androgenetic zygotes to the high hydrostatic pressure shock (51.711 MP a for 4 min) applied 420 min after insemination. Androgenetic larvae that hatched from the brook charr and the hybrid eggs were shown to be homozygous brook charr individuals. Androgenetic individuals exhibited 84 chromosomes and 100 chromosome arms ( FN ), values characteristic for the brook charr diploid cells. Strategy hybridize first than induce androgenesis should be tested in order to provide androgenetic offspring in other salmonids that are able to cross and produce fertile offspring.