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The Origin of Amniotic Polymorphonuclear Leucocytes in the Mare
Author(s) -
Hemberg E,
Einarsson S,
Jones B,
Mikko S
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12237
Subject(s) - amniotic fluid , andrology , fetus , staining , biology , aborted fetus , pregnancy , pathology , medicine , genetics
Contents The objective of this study was to investigate the presence and origin of polymorphonuclear leucocytes (PMNLs) in the amniotic fluid of mares giving birth to healthy foals. Material from 25 mares was included. Amniotic fluid was collected during parturition before breakage of the amniotic vesicle. Manual microscopic cytologic evaluation was made on cytospin preparations after staining. PMNL s were found in all amniotic samples examined. The genomic DNA was extracted from 12 of the amniotic fluid samples and was genotyped. The results indicate that the PMNL s originate from the foetus.