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I mmunolocalization of M elatonin and F ollicle‐ S timulating H ormone R eceptors in C aprine O varies and their E ffects D uring in vitro D evelopment of I solated P re‐ A ntral F ollicles
Author(s) -
Barros VRP,
Cavalcante AYP,
Macedo TJS,
Barberino RS,
Lins TLB,
Gouveia BB,
Menezes VG,
Queiroz MAA,
Araújo VR,
Palheta RC,
Leite MCP,
Matos MHT
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12209
Subject(s) - melatonin , antral follicle , oocyte , endocrinology , medicine , andrology , biology , in vitro , hormone , chemistry , embryo , biochemistry , microbiology and biotechnology
Contents The expression of melatonin type 1 ( MT 1) and FSH ( FSHR ) receptors in caprine ovaries and the effects of these hormones on the in vitro development of isolated pre‐antral follicles were evaluated. Follicles (≤200 μm) were cultured for 12 days in α ‐ MEM (control) or melatonin (100 or 1000 pg/ml) or sequential melatonin medium (100 pg/ml: from day 0 to day 6; 1000 pg/ml: from day 6 to day 12; experiment 1) and in control or sequential FSH (100 ng/ml from day 0 to day 6; 500 ng/ml from day 6 to day 12) or sequential melatonin or this latter plus sequential FSH (experiment 2). MT 1 and FSHR expressions were observed in granulosa cells from secondary and antral follicles. The oocytes from primordial and primary follicles also express FSHR . Sequential melatonin increased the percentage of normal follicles and oocyte recovery compared with the control or melatonin (1000 pg/ml) at day 12. In experiment 2, all the treatments increased the normal follicles and growth compared with the control. In conclusion, this study demonstrated the presence of MT 1 and FSHR in caprine ovaries. The addition of increased concentrations of melatonin (sequential medium) or FSH can be used to promote the in vitro development of caprine pre‐antral follicles.