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Acid–Base Parameters and Steroid Concentrations in Pre‐Ovulatory Follicles and Plasma of Lactating Dairy Cows with Spontaneous and Synchronized Oestrus or Follicular Cyst
Author(s) -
Hussein HA,
Boryczko Z,
Bostedt H
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12171
Subject(s) - estrous cycle , follicular phase , follicular fluid , medicine , endocrinology , chemistry , zoology , andrology , biology , oocyte , embryo , microbiology and biotechnology
Contents The study aimed to compare the acid–base balance and steroid concentrations between follicular fluids ( FF ) of pre‐ovulatory follicles derived from a spontaneous oestrus ( SO ), synchronized or induced oestrus ( IO ) and follicular cysts ( CYS ) and between FF and blood in dairy cows. Forty‐two dairy cows were included in this study. The animals were allocated to three groups: SO (n = 23); IO (n = 11) using GnRH at day 0 and day 9 and PGF 2 α at day 7; and animals with CYS (n = 10). The follicular fluids ( FF ) were aspirated from the cyst/pre‐ovulatory follicles (∅ ≥ 15 mm) after SO and after second GnRH dose in IO by transvaginal ultrasound‐guided ovum pick‐up technique. Blood samples ( BL ) were collected in heparinized vacutainer tubes. The oxygen tension ( pO 2) in FF of IO was higher (p < 0.05) than in SO and CYS groups. There were negative correlations (p < 0.001, r = −0.89) between FF and blood pO 2. The carbon dioxide tension ( pCO 2) and lactate level in FF of CYS group were higher (p < 0.05) than in SO and IO groups. There were negative correlations (p < 0.01, r = −0.73) between blood and FF pO 2. Oestradiol‐17β concentration in pre‐ovulatory follicles and plasma of the SO group was higher (p < 0.001) than in IO and CYS groups. Progesterone concentration in pre‐ovulatory follicles and plasma of the SO and IO groups was lower (p < 0.01) than in CYS group. Plasma androstenedione concentration in SO and IO groups was higher (p < 0.05) than in CYS group. In conclusion, acid–base parameters, E 2 and P 4 levels in the follicular fluid of both IO and CYS groups were deviated greatly from the physiological level (disturbances of intrafollicular/intracystic environment), which may affect the quality of both the oocyte and the granulosa cells.