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Differential Ovarian Morphometry and Follicular Expression of BMP 15, GDF 9 and BMPR 1B Influence the Prolificacy in Goat
Author(s) -
Pramod RK,
Sharma SK,
Singhi A,
Pan S,
Mitra A
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12165
Subject(s) - bone morphogenetic protein 15 , follicular phase , biology , growth differentiation factor 9 , ovulation , antral follicle , andrology , breed , corpus luteum , ovary , endocrinology , gene , zoology , folliculogenesis , bone morphogenetic protein , genetics , pregnancy , lactation , medicine , hormone , bone morphogenetic protein 7
Contents The variation in the kidding size of Black Bengal and Sirohi breed of goats makes them an interesting genetic material to study the underlying genetic mechanism of prolificacy. Accordingly, we studied the comparative ovarian morphometry including disparity in numbers of antral follicles of different sizes between these two breeds. Further, we evaluated the differential expression of the important candidate genes (viz., BMP 15, GDF 9 and BMPR 1 B ) known to influence the ovulation rates and the prolificacy. The ovaries of Black Bengal (n = 20) goat were lighter (p < 0.01) in weight and smaller (p < 0.01) in diameter than those of Sirohi (n = 19) goats but possessed more numbers (p < 0.05) of corpus luteum (CL), large and small antral follicles. Quantitative real‐time PCR (RT‐ qPCR ) analysis revealed differential expression of mRNA s encoding for the BMP15 and GDF9. Small antral follicles of B lack B engal goats expressed 2.78‐fold more (p < 0.05) of BMP 15 than those of S irohi goat. Expression of BMP 15 (p < 0.01) and GDF 9 (p < 0.05) mRNA s was more abundant in the small than the large antral follicles of B lack B engal goat. The more numbers of antral follicles per unit of ovarian mass and differential expression of BMP 15 and GDF 9 may serve as an important clue for higher prolificacy.