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Transcription Profile of Candidate Genes for the Acquisition of Competence During Oocyte Growth in Cattle
Author(s) -
Bessa IR,
Nishimura RC,
Franco MM,
Dode MAN
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12162
Subject(s) - oocyte , biology , antral follicle , folliculogenesis , gene , andrology , gene expression , oogenesis , genetics , follicular phase , embryogenesis , embryo , medicine
Contents The aim of this study was to investigate the expression profile of candidate genes involved in competence during oocyte growth. The candidate genes ( BMP 15, OOSP 1, H1FOO , H 2A, H 3A, H 4, SLBP , DNMT 1, DNMT 3B, HAT 1, HDAC 2 and SUV 39H1 ) were selected because of their possible involvement in determining oocyte developmental competence. Pre‐antral and antral follicles were isolated from the ovaries of Z ebu ( B os indicus ) cows, measured and classified into the following categories according to their diameter: (i) oocytes from primordial follicles: diameter <20 μm, (ii) oocytes from primary follicles: 25–35 μm, (iii) oocytes from small secondary follicles: 40–60 μm, (iv) oocytes from large secondary follicles: 65–85 μm, (v) oocytes from small antral follicles: 100–120 μm, and (vi) oocytes from large antral follicles: >128 μm. Total RNA was extracted from four pools of 25 oocytes for each category of follicles, and the genes were quantified by qPCR . Target gene expression was normalized using the gene PPIA . The results suggest that stocks of the studied transcript genes accumulate before the final phase of folliculogenesis. The HDAC 2 gene was the only gene in which a differential expression was observed at stage associated with competence acquisition.