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Comparison of Different Glycerol and Egg Yolk Concentrations Added to T ris‐based Extender for the Collared Peccaries ( T ayassu tajacu ) Semen Freezing
Author(s) -
Alves HM,
Oliveira IRS,
Castelo TS,
Lima GL,
Souza ALP,
Moreira MAP,
Paula VV,
Silva AR
Publication year - 2013
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12115
Subject(s) - extender , yolk , semen , glycerol , electroejaculation , sperm , sperm motility , biology , andrology , zoology , chemistry , food science , anatomy , biochemistry , botany , medicine , organic chemistry , polyurethane
Contents This study aimed to evaluate various concentrations of egg yolk (5, 10, or 20%) in combination with different concentrations of glycerol (3% or 6%) added to a T ris‐based extender on the post‐thaw characteristics of sperm obtained from Tayassu tajacu . For this purpose, semen from 10 sexually male mature collared peccaries was collected by electroejaculation and evaluated for sperm motility, vigour, viability, morphology and functional membrane integrity. The ejaculates were initially extended in T ris‐fructose plus egg yolk (5%, 10% or 20%). After cooling, the semen was added to T ris‐egg yolk plus glycerol (6% or 12%), resulting in a final concentration of 3% or 6% glycerol of the extender. Straws were frozen using liquid nitrogen and thawed in a water bath at 37°C for 30 s. The frozen–thawed semen was evaluated as reported for fresh semen. After thawing, a significant decrease was verified for sperm motility and vigour, for all the samples in comparison with fresh semen. However, no differences were evidenced among treatments for any sperm characteristics evaluated (p > 0.05), except for the combination between 10% egg yolk and 6% glycerol, which provided the worst preservation of functional membrane integrity (p < 0.05). The interactions between higher concentrations of egg yolk (20%) and glycerol (6%) and also between lower concentrations of the same substances (5% egg yolk and 3% glycerol) added to the Tris‐based extender negatively affected the preservation of the normal sperm morphology after thawing (p < 0.05). In conclusion, the use of Tris‐based extender added to 10% or 20% egg yolk plus 3% glycerol is recommended for effective sperm cryopreservation in collared peccaries.