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Species‐specific Challenges in Dog Cloning
Author(s) -
Kim GA,
Oh HJ,
Park JE,
Kim MJ,
Park EJ,
Jo YK,
Jang G,
Kim MK,
Kim HJ,
Lee BC
Publication year - 2012
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12035
Subject(s) - somatic cell nuclear transfer , cloning (programming) , computational biology , biology , somatic cell , microbiology and biotechnology , genetics , embryo , computer science , gene , blastocyst , embryogenesis , programming language
Contents Somatic cell nuclear transfer (SCNT) is now an established procedure used in cloning of several species. SCNT in dogs involves multiple steps including the removal of the nuclear material, injection of a donor cell, fusion, activation of the reconstructed oocytes and finally transfer to a synchronized female recipient. There are therefore many factors that contribute to cloning efficiency. By performing a retrospective analysis of 2005–2012 published papers regarding dog cloning, we define the optimum procedure and summarize the specific feature for dog cloning.