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Effects of Cooling Time on Membrane Integrity and Motility of Frozen‐Thawed Canine Spermatozoa using Two Different Commercial Egg Yolk–based Extenders at Two Different Cooldown Equilibration Times
Author(s) -
du Bois S,
Len JA,
Parlevliet JM,
Eilts BE
Publication year - 2012
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12018
Subject(s) - semen , extender , sperm motility , semen extender , sperm , andrology , motility , yolk , biology , chemistry , zoology , food science , medicine , organic chemistry , polyurethane , genetics
Contents Two commercially available egg yolk–based semen extenders, one marketed for human semen freezing ( HEYE ) and one marketed for canine semen freezing ( CEYE ), were used to cryopreserve semen from single ejaculates of 11 different dogs. For each extender, a 30‐ and a 60–min cooldown period was used prior to the addition of the extender containing glycerol and then immediately frozen in liquid nitrogen vapours. Sperm motility was measured using a computer‐assisted semen analysis ( CASA ) system. Sperm intact membranes were measured using SYBER ‐14 and propidium iodide. Semen in the HEYE cooled for 60 min had a significantly greater percentage of intact membranes than the semen in the HEYE cooled for 30 min (p = 0.02). Semen in the HEYE cooled for 60 min had significantly greater total motility (p = 0.007) and progressive motility (p = 0.004) than semen cooled for 60 min in the CEYE and semen cooled for 30 min in the HEYE (total motility p = 0.02 and progressive motility p = 0.02). Semen cooled for 60 min in the CEYE did not differ significantly in total (p = 0.6) or progressive motility (p = 0.4) than semen cooled for 30 min in the CEYE. There was no difference in total (p = 0.8) or progressive motility (p = 0.8) between the semen cooled for 30 min in the HEYE and the semen cooled for 30 min in the CEYE .