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Canine Fibroblasts Expressing Human Transcription Factors: What is in the Route for the Production of Canine Induced Pluripotent Stem Cells
Author(s) -
Gonçalves NJN,
Bressan FF,
Souza A,
Martins DS,
Miglino MA,
Meirelles FV,
Perecin F,
Ambrósio CE
Publication year - 2012
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/rda.12002
Subject(s) - biology , induced pluripotent stem cell , somatic cell , microbiology and biotechnology , fibroblast , transcription (linguistics) , transgene , cell culture , transcription factor , stem cell , embryonic stem cell , reporter gene , gene , gene expression , genetics , linguistics , philosophy
Contents The aim of this study was to further clarify the mechanisms involved in inducing pluripotency using canine foetal fibroblast cells. The two pluripotency‐related transcription factors, OCT 4 and SOX 2, coupled to a fluorescent reporter gene were transduced, individually or in combination, using a lentiviral system. Stable transgenic cell lineages were obtained and canine cells showed to be highly responsive to the integration and expression of human SOX 2 and OCT 4, also depending on the amount of virus used for incubation. Such positive results are essential for the establishment of pluripotency induction through the incorporation of known transcription factors into the genome of somatic cells.