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RNA interference in shrimp and potential applications in aquaculture
Author(s) -
Nguyen Dung Viet,
Christiaens Olivier,
Bossier Peter,
Smagghe Guy
Publication year - 2018
Publication title -
reviews in aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.998
H-Index - 47
eISSN - 1753-5131
pISSN - 1753-5123
DOI - 10.1111/raq.12187
Subject(s) - rna , rna interference , rna silencing , aquaculture , biology , computational biology , non coding rna , gene expression , gene , gene silencing , microbiology and biotechnology , genetics , fish <actinopterygii> , fishery
As the RNA interference mechanism was discovered, researchers have made huge strides in unravelling biological mechanisms at the gene level in a wide range of organisms. RNA i is a simple and rapid method that allows silencing gene expression at the post‐transcriptional level and is triggered by the introduction of double‐stranded RNA into the cell, leading to the degradation of the corresponding mRNA . In this review, we will provide an overview of successful RNA i experiments to date in Crustacea, and shrimp in particular, with special focus on various potential applications of RNA interference in research and aquaculture. An important aspect of RNA i in any species is the delivery of the double‐stranded RNA and the barriers that have to be overcome to get the ds RNA into the target cells. Different options, experimental procedures, success rates and challenges in using RNA i are discussed in this review.