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Proliferating cell nuclear antigen and V asa protein expression during gonadal development and sexual differentiation in cultured S iberian ( A cipenser baerii B randt, 1869) and R ussian ( A cipenser gueldenstaedtii B randt & R atzeburg, 1833) sturgeon
Author(s) -
Rzepkowska Malgorzata,
Ostaszewska Teresa
Publication year - 2014
Publication title -
reviews in aquaculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.998
H-Index - 47
eISSN - 1753-5131
pISSN - 1753-5123
DOI - 10.1111/raq.12027
Subject(s) - biology , gonad , development of the gonads , meiosis , germ cell , proliferating cell nuclear antigen , gametogenesis , mitosis , ovary , sexual differentiation , microbiology and biotechnology , testicle , andrology , embryo , endocrinology , genetics , cell growth , embryogenesis , gene , medicine
Early gonadal development in R ussian ( A cipenser gueldenstaedtii B randt, 1869) and S iberian sturgeon ( A cipenser baerii Brandt & R atzeburg, 1833) reared in controlled conditions from dph 1–439 (days post hatching) was studied using histological methods. Migrating primordial germ cells ( PGC s) were observed in both species on dph 1. On dph 25 in both species gonad primordia developed in gonadal ridges. In dph 115 juveniles two types of gonad were distinguished, putative ovary and putative testicle. Meiotic activity was observed in ovaries on dph 197, while in testicles the first meiotic divisions were observed much later, on dph 439 and only in several S iberian specimens. The presence of V asa protein was confirmed exclusively in germ cells during gonadogenesis, indicating that V asa is a good marker of germinal cells also in A cipenseridae. Vasa protein displayed a nuage association or nuage‐like localization. Some differences in the level of V asa protein expression during male and female gonad development were also observed. In the gonads of both investigated sturgeon species the PCNA (proliferating cell nuclear antigen)‐positive reaction was observed first in oogonia, while in spermatogonia at approximately 115 dph which was correlated with active mitotic divisions of germ cells. Surprisingly, meiotic oocytes arrested in diplotene also demonstrated strong PCNA ‐positive germinal vesicle.