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Characterization of Porphyra lucasii pigment mutant induced by gamma irradiation
Author(s) -
Lee HakJyung,
Choi Jongil,
Kim Gwang H.
Publication year - 2018
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/pre.12216
Subject(s) - biology , pigment , rapd , porphyra , mutant , antioxidant , irradiation , chlorophyll , strain (injury) , photosynthetic pigment , gel electrophoresis , somatic cell , botany , photosynthesis , proteome , gamma irradiation , biochemistry , microbiology and biotechnology , gene , algae , genetic diversity , anatomy , chemistry , population , demography , physics , organic chemistry , sociology , nuclear physics
SUMMARY The present study describes the isolation of a pigment mutant strain of Porphyra lucasii , which was obtained using gamma irradiation. Gametophytic blades of this seaweed were irradiated with gamma rays at doses of 500 and 1000 Gy. After irradiation, red color was observed in the blades exposed to the 500 Gy dose. Single somatic cells from these irradiated blades were isolated from archespores and then cultured to obtain pigment mutant blades that are referred to as 500GR in this investigation. Evaluation of genetic variations in these blades was performed using random amplification of polymorphic DNA (RAPD) analysis. Chlorophyll content, antioxidant activity, and total phenolic content were found to be higher in 500GR than in the wild type (WT) algal strain. Protein extracts obtained from the blades were examined using 2D gel electrophoresis; changes in the proteome were finally analyzed using MS/MS. A total of 216 proteins were detected. From these, nine proteins were over‐expressed in the pigment spots in 500GR as compared to WT. These results indicate that the over‐expressed proteins are either antioxidant enzymes or photosynthesis‐related proteins.