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Lipid compositions in diatom Conticribra weissflogii under static and aerated culture conditions
Author(s) -
Li Yanrong,
Ye Mengwei,
Zhang Runtao,
Xu Jilin,
Zhou Chengxu,
Yan Xiaojun
Publication year - 2016
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/pre.12144
Subject(s) - thalassiosira weissflogii , biology , docosahexaenoic acid , biochemistry , food science , aeration , diatom , eicosapentaenoic acid , phosphatidylethanolamine , fatty acid , phospholipid , polyunsaturated fatty acid , botany , phosphatidylcholine , phytoplankton , ecology , membrane , nutrient
SUMMARY The diatom Conticribra weissflogii is a microalga with high nutrition value, rich in docosahexaenoic acids ( DHA ) and eicosapentaenoic acids ( EPA ). In order to study the effect of culture conditions on the changes of lipid compositions, the intact lipid structural profiles and fatty acids in C. weissflogii were monitored under static and aerated culture conditions. The results showed that, lipids identified in C. weissflogii were neutral lipid triacylglycerols ( TAG ), betaine lipid diacylglycerylcarboxy‐N‐hydroxymethyl‐choline ( DGCC ), phosphatidylcholine ( PC ) and four classes of photosynthetic glycerolipids. The profiles of lipid metabolites of C. weissflogii were different between two culture modes, with the following characteristics under aerated conditions: TAG s increased significantly, whereas the levels of sulfoquinovosyl diacylglycerol ( SQDG ), monogalactosyldiacylglycerol ( MGDG ), and DGCC decreased. Furthermore, higher contents of EPA ‐rich TAG and EPA/DHA ‐rich DGCC were detected at the end of stationary phase, while EPA/DHA ‐rich PC , EPA ‐rich MGDG and EPA ‐rich digalactosyldiacylglycerol ( DGDG ) were obtained in the exponential phase under static conditions. Meanwhile, the contents of almost all classes of the essential fatty acids ( EFA s)‐enriched lipids increased at onset of stationary phase under aerated conditions. Taken together, given that the high levels of EFA s are required for artificial rearing of marine organisms, aeration is critically important for increasing the production rate and the contents of EFA molecules and therefore increasing the nutritional value of the microalgae.