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Delimitation of cryptic species of the S cytosiphon lomentaria complex ( S cytosiphonaceae, P haeophyceae) in J apan, based on mitochondrial and nuclear molecular markers
Author(s) -
Kogame Kazuhiro,
Ishikawa Shozo,
Yamauchi Kei,
Uwai Shinya,
Kurihara Akira,
Masuda Michio
Publication year - 2015
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/pre.12091
Subject(s) - biology , phylogenetic tree , haplogroup , haplotype , mitochondrial dna , species complex , gene , botany , genetics , genotype
Summary S cytosiphon lomentaria ( S cytosiphonaceae, E ctocarpales) is believed to include some cryptic species, particularly in the P acific. We attempted to delimit these species in J apan using mitochondrial cox 1 and cox 3 and nuclear ITS2 and the second intron of the centrin gene ( cetn‐ int2). Fifty‐three cox 1+ cox 3 mitotypes, 26 ITS2 ribotypes and 45 cetn‐ int2 haplotypes were found in 107 samples collected from 33 localities in J apan. Based on phylogenetic analyses, similar sequence types were grouped into ten mitogroups, eight ribogroups and six cetn‐ int2 haplogroups (sequence‐type groups). From the molecular trees and combinations of the mito‐, ribo‐ and haplogroups, three cryptic species were apparent (Groups I–III). Group I, widely distributed on P acific coasts, was highly supported by all molecular trees, whereas G roups II ( N orth P acific) and III ( N orthwestern P acific and A ustralasia) were more closely related to each other. However, sequence‐type‐group combinations that would be characteristic of hybrids between Groups II and III were not detected, suggesting no gene flow between the two Groups. Further investigations of an additional 127 sympatrically growing plants supported the absence of gene flow between Groups II and III. Four samples did not belong to any of the Groups I–III and possibly represent additional species.