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Occurrence and induction of a ultraviolet‐absorbing substance in the cyanobacterium F ischerella muscicola TISTR8215
Author(s) -
Rastogi Rajesh P.,
Incharoensakdi Aran
Publication year - 2015
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/pre.12069
Subject(s) - photosynthetically active radiation , biology , absorption (acoustics) , ultraviolet radiation , fluorescence , radiation , ultraviolet , botany , irradiation , chemistry , photosynthesis , materials science , optics , optoelectronics , physics , radiochemistry , nuclear physics
Summary The filamentous cyanobacterium F ischerella muscicola TISTR8215 was tested for the presence of ultraviolet ( UV )‐absorbing mycosporine‐like amino acids ( MAA s) and their induction by UV radiation. Reverse‐phase high performance liquid chromatographic coupled with photodiode‐array detection studies revealed the presence of a MAA having an absorption maximum at 332 nm and a retention time of around 16.1 min. Based on absorption maximum, the compound was designated as M ‐332. This is the first report for the occurrence of a MAA and its inducibility as influenced by UV radiation in F ischerella strains studied so far. Photosynthetically active radiation ( PAR ) had no significant impact on MAA induction. PAR + UV ‐ A radiation significantly induced the synthesis of M ‐332; however, PAR + UV ‐ A + UV ‐ B radiation conferred highest impact on MAA synthesis. The cultures exposed to alternate light and dark conditions showed the induction of M ‐332 synthesis mostly during the light period in contrast to the decreased levels of M ‐322 during the dark period suggesting a circadian induction of its synthesis. Overall results indicate that F . muscicola may protect itself from deleterious short wavelength UV radiation by synthesizing the photoprotective compounds particularly during summer time in its natural brightly‐lit habitats.