Premium
Phylogenetic position and morphology of lichenized Trentepohliales ( U lvophyceae, C hlorophyta) from selected species of G raphidaceae
Author(s) -
Hametner Christina,
StockerWörgötter Elfie,
Rindi Fabio,
Grube Martin
Publication year - 2014
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1111/pre.12055
Subject(s) - biology , phylogenetic tree , lichen , algae , botany , phylogenetics , gene , genetics
Summary The order T rentepohliales comprises a diverse and widespread group of green algae in temperate and tropical regions. The free‐living representatives of this order have been investigated in more detail, whereas less is known about the trentepohlialean photobionts of lichens. Selected lichen species of the family G raphidaceae ( G raphis propinqua , G raphis scripta , G raphis submarginata ) from different climatic regions were chosen for the isolation of trentepohlialean photobionts. Three different nutrient media (modified B old's B asal medium, modified W oods H ole MBL medium, optimal H aematococcus medium) were tested for the cultivation of these algae. The differences of the growth reactions of the isolated photobionts in culture were studied examining morphological characteristics, reproductive strategies and variations in carotenoid accumulation. The phylogenetic positions of the photobionts were investigated using sequences of the 18S rRNA gene, ITS region, and rbc L gene. Three photobiont strains were identified as P rintzina lagenifera and one as T rentepohlia sp. according to the phylogenetic data and the morphological observations under culture conditions.