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Genome‐wide association mapping identifies HvNIP2;2 / HvLsi6 accounting for efficient boron transport in barley
Author(s) -
Jia Zhongtao,
Bienert Manuela Désirée,
von Wirén Nicolaus,
Bienert Gerd Patrick
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13340
Subject(s) - biology , hordeum vulgare , quantitative trait locus , hordeum , plant genetics , genetic variation , gene , genetics , shoot , genome , botany , poaceae
Boron (B) is an essential mineral element for plant growth, and the seed B pool of crops can be crucial when seedlings need to establish on low‐B soils. To date, it is poorly understood how B accumulation in grain crops is genetically controlled. Here, we assessed the genotypic variation of the B concentration in grains of a spring barley ( Hordeum vulgare L.) association panel that represents broad genetic diversity. We found a large genetic variation of the grain B concentration and detected in total 23 quantitative trait loci (QTLs) using genome‐wide association mapping. HvNIP2;2/HvLsi6 , encoding a potential B‐transporting membrane protein, mapped closely to a major‐effect QTL accounting for the largest proportion of grain B variation. Based on transport studies using heterologous expression systems and gene expression analysis, we demonstrate that HvNIP2;2/HvLsi6 represents a functional B channel and that expression variation in its transcript level associates with root and shoot B concentrations as well as with root dry mass formation under B‐deficient conditions.