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Linking changes in chlorophyll a fluorescence with drought stress susceptibility in mung bean [ Vigna radiata (L.) Wilczek]
Author(s) -
Bano Hussan,
Athar HabiburRehman,
Zafar Zafar Ullah,
Kalaji Hazem M.,
Ashraf Muhammad
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13327
Subject(s) - vigna , photosystem ii , chlorophyll fluorescence , chemistry , horticulture , photosynthesis , quantum yield , drought stress , radiata , cultivar , fluorescence , chlorophyll , botany , biology , biochemistry , physics , quantum mechanics
In the present study, the mung bean cv. NM‐13‐1 Tol was selected as drought‐tolerant and NM‐54 Sens as drought‐sensitive. The effects of progressive drought (16 days) on the photosystem II (PSII) activity was assessed using OJIP and JIP‐test in the selected two mung bean cultivars differing in drought tolerance. Drought stress reduced the relative water content to 70% (at threshold) and 62% (below the threshold) in cv.NM‐13‐1 Tol and NM‐54 sens , respectively. The greater reduction in quantum yield of PSII in cv.NM‐54 sens due to drought stress was due to PSII photodamage. Raw OJIP induction curves and F o and F m normalised curves showed that significant changes in fluorescence occurred at the O, J, I and P steps only in cv. NM‐54 sens . Double normalised differential kinetics indicated adverse effects at the antennae, oxygen‐evolving complex and intersystem electron acceptors in cv.NM54 sens . Moreover, JIP‐test analysis showed that drought stress caused a greater decrease in performance index (PI ABS ) in cv.NM‐54 sens as compared to that in cv. NM‐13‐1 Tol , which is associated with an increase in V j , rate of accumulation of closed reaction centres (M o ), energy fluxes for absorption (ABS/RC), trapping (TR o /RC), electron transport (ET o /RC), and dissipation of absorbed energy as heat (DI o /RC). In conclusion, two‐week drought stress reduced the RWC below the threshold in cv.NM54 sens , which resulted in damages at the donor and acceptor sides of PSII. However, cv.NM‐13‐1 Tol somehow maintained the RWC around the threshold and thus protected PSII. Of various JIP‐test parameters, PI ABS , F v /F m , V j and M o are key indicators of drought stress tolerance in mung bean cultivars.