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Transcriptome analysis reveals key genes regulating signaling and metabolic pathways during the growth of moso bamboo ( Phyllostachys edulis ) shoots
Author(s) -
Lan Yangang,
Wu Lin,
Wu Min,
Liu Huanlong,
Gao Yameng,
Zhang Kaimei,
Xiang Yan
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13296
Subject(s) - phyllostachys edulis , biology , cytokinin , shoot , transcriptome , metabolic pathway , signal transduction , sugar , bamboo , plant hormone , bamboo shoot , botany , biochemistry , microbiology and biotechnology , gene , gene expression , auxin , ecology , raw material
Moso bamboo ( Phyllostachys edulis ), a high‐value bamboo used to produce food (young shoots), building, and industrial goods. To explore key candidate genes regulating signal transduction and metabolic processes during the initiation of stem elongation in moso bamboo, a transcriptome analysis of the shoots during three successive early elongation stages was performed. From cluster and differential expression analyses, 2984 differentially expressed genes (DEGs) were selected for an enrichment analysis. The DEGs were significantly enriched in the plant hormone signal transduction, sugar and starch metabolism, and energy metabolism pathways. Consequently, the DEG expression patterns of these pathways were analyzed, and the plant endogenous hormone and carbon metabolite (including sucrose, total soluble sugar, and starch) contents for each growth stage, of the shoot, were determined. The cytokinin‐signaling pathway was continuously active in the three successive elongation stages, in which several cytokinin‐signaling genes played indispensable roles. Additionally, many key DEGs regulating sugar, starch metabolism, and energy conversion, which are actively involved in energy production and substrate synthesis during the continuous growth of the shoots, were found. In summary, our study lays a foundation for understanding the mechanisms of moso bamboo growth and provides useful gene resources for breeding through genetic engineering.