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Ecotopic over‐expression of PoCHS from Paeonia ostii altered the fatty acids composition and content in Arabidopsis thaliana
Author(s) -
Peng LiPing,
Hao Qing,
Men SiQi,
Wang XiRuo,
Huang WenYuan,
Tong NingNing,
Chen Mo,
Liu ZhengAn,
Ma XiaoFeng,
Shu QingYan
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13293
Subject(s) - arabidopsis , chalcone synthase , biochemistry , gene , ectopic expression , arabidopsis thaliana , biology , flavonoid biosynthesis , enzyme , biosynthesis , function (biology) , fatty acid , gene expression , genetics , mutant , transcriptome
Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthetic pathway and has been studied in many plants, but the function of the CHS gene has not been well characterized in Paeonia ostii . In this study, we obtained a CHS homolog gene from P. ostii , which possessed the putative conserved amino acids of chalcone synthase by multiple alignment analysis and demonstrated the highest expression in developing seeds. In vitro assays of the recombinant PoCHS protein confirmed enzymatic activity using malonyl‐CoA and 4‐coumaroyl‐CoA as substrates, and the optimal pH and reaction temperature were 7.5 and 40 °C, respectively. Furthermore, ectopic over‐expression of PoCHS in Arabidopsis up‐regulated the expression levels of genes involved in seed development ( ABI ), glycolysis ( PKp2 , PDH‐E1a , and SUS2/3 ), and especially fatty acid biosynthesis ( BCCP2 , CAC2 , CDS2 , FatA , and FAD3 ). This resulted in an increased unsaturated fatty acid content, especially α‐linolenic acid, in transgenic Arabidopsis seeds. In this study, we examined the functions of CHS homolog of P. ostii and demonstrated its new function in seed fatty acid biosynthesis.