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Importance of inositols and their derivatives in cowpea under root dehydration: An omics perspective
Author(s) -
FerreiraNeto José R. C.,
Silva Manassés D.,
Rodrigues Fabiana A.,
Nepomuceno Alexandre L.,
Pandolfi Valesca,
Lima Morais David A.,
Kido Ederson A.,
BenkoIseppon Ana M.
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13292
Subject(s) - phosphatidylinositol , biochemistry , biology , phosphatase , raffinose , inositol , kinase , phospholipase c , enzyme , receptor , sucrose
This work presents a robust analysis of the inositols (INSs) and raffinose family oligosaccharides (RFOs) pathways, using genomic and transcriptomic tools in cowpea under root dehydration. Nineteen (~70%) of the 26 scrutinized enzymes presented transcriptional up‐regulation in at least one treatment time. The transcriptional orchestration allowed categorization of the analyzed enzymes as time‐independent (those showing the same regulation throughout the assay) and time‐dependent (those showing different transcriptional regulation over time). It is suggested that up‐regulated time‐independent enzymes (INSs: myo‐inositol oxygenase, inositol‐tetrakisphosphate 1‐kinase 3, phosphatidylinositol 4‐phosphate 5‐kinase 4‐like, 1‐phosphatidylinositol‐3‐phosphate 5‐kinase, phosphoinositide phospholipase C, and non‐specific phospholipase C; RFOs: α‐galactosidase, invertase, and raffinose synthase) actively participate in the reorganization of cowpea molecular physiology under the applied stress. In turn, time‐dependent enzymes, especially those up‐regulated in some of the treatment times (INSs: inositol‐pentakisphosphate 2‐kinase, phosphatidylinositol 4‐kinase, phosphatidylinositol synthase, multiple inositol polyphosphate phosphatase 1, methylmalonate‐semialdehyde dehydrogenase, triosephosphate isomerase, myo‐inositol‐3‐phosphate synthase, phosphatidylinositol 3,4,5‐trisphosphate 3‐phosphatase and protein‐tyrosine‐phosphatase, and phosphatidylinositol 3‐kinase; RFOs: galactinol synthase) seem to participate in fine‐tuning of the molecular physiology, helping the cowpea plants to acclimatize under dehydration stress. Not all loci encoding the studied enzymes were expressed during the assay; most of the expressed ones exhibited a variable transcriptional profile in the different treatment times. Genes of the INSs and RFOs pathways showed high orthology with analyzed Phaseoleae members, suggesting a relevant role within this legume group. Regarding the promoter regions of INSs and RFOs genes, some bona fide cis ‐regulatory elements were identified in association with seven transcription factor families (AP2‐EFR, Dof‐type, MADS‐box, bZIP, CPP, ZF‐HD, and GATA‐type). Members of INSs and RFOs pathways potentially participate in other processes regulated by these proteins in cowpea.