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Comparative physiological and proteomic analysis deciphering tolerance and homeostatic signaling pathways in chrysanthemum under drought stress
Author(s) -
Sahithi Bhuma Mani,
Razi Kaukab,
Al Murad Musa,
Vinothkumar Avanthika,
Jagadeesan Saravanan,
Benjamin Lincy Kirubhadharsini,
Jeong Byoung Ryong,
Muneer Sowbiya
Publication year - 2021
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13142
Subject(s) - stomatal conductance , biology , transpiration , photosynthesis , metabolic pathway , drought tolerance , plant physiology , reactive oxygen species , botany , proteome , microbiology and biotechnology , metabolism , biochemistry
Drought is increasing prevalently, mostly due to global warming, and harmful effects associated with drought stress include a reduction in the developmental phases of the plant life cycle. Drought stress affects vital metabolic processes in plants such as transpiration, photosynthesis and respiration. The other physiological and cellular processes like protein denaturation and aggregation are also affected by drought. Drought stress severely affects the floral industry by reducing the yield of flowers and among them is chrysanthemum ( Dendranthema grandiflorum ). In this study, we determined the critical signaling pathways, tolerance mechanism and homeostatic maintenance to drought stress in chrysanthemum. We compared the proteome of chrysanthemum leaves under drought stress. Among 250 proteins on 2DE gels, 30 protein spots were differentially expressed. These proteins were involved in major signaling pathways including, stress response, flower development and other secondary metabolism like physiological transport, circadian rhythm, gene regulation, DNA synthesis and protein ubiquitination. A reduction in a biomass, flower development, photosynthesis, transpiration, stomatal conductance, PSII yield and stomatal index was also observed in our results. Moreover, the stress markers and leaf water potential were also analyzed to depict the level of stress tolerance in chrysanthemum. Our data suggested that chrysanthemum plants developed reactive oxygen species and revealed signaling pathways to cope with drought stress. These results, thus, provide crucial information about how chrysanthemum plants respond to drought stress to maintain homeostasis.