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Involvement of nitrate reductase‐dependent nitric oxide production in magnetopriming‐induced salt tolerance in soybean
Author(s) -
Kataria Sunita,
Jain Meeta,
Tripathi Durgesh K.,
Singh Vijay P.
Publication year - 2020
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13031
Subject(s) - nitrate reductase , nitric oxide , sodium nitroprusside , germination , chemistry , seedling , hydrogen peroxide , nitric oxide synthase , horticulture , biochemistry , food science , enzyme , biology , organic chemistry
In the present study, experiments were performed to investigate the role of nitric oxide (NO) in magnetopriming‐induced seed germination and early growth characteristics of soybean ( Glycine max ) seedlings under salt stress. The NO donor (sodium nitroprusside, SNP), NO scavenger (2‐[4‐carboxyphenyl]‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide, CPTIO), inhibitors of nitrate reductase (sodium tungstate, ST) or NO synthase (N‐nitro‐L‐Arg‐methyl ester, LNAME) and NADPH oxidase inhibitor (diphenylene iodonium, DPI) have been used to measure the role of NO in the alleviation of salinity stress by static magnetic field (SMF of 200 mT, 1 h). Salt stress (50 mM NaCl) significantly reduced germination and early growth of seedlings emerged from non‐primed seeds. Pre‐treatment of seeds with SMF positively stimulated the germination and consequently promoted the seedling growth. ST, LNAME, CPTIO and DPI significantly decreased the growth of seedling, activities of α‐amylase, protease and nitrate reductase (NR), hydrogen peroxide (H 2 O 2 ), superoxide (O 2 •− ) and NO content in roots of seedlings emerged from non‐primed and SMF‐primed seeds. However, the extent of reduction was higher with ST in seedlings of SMF‐primed seeds under both conditions, whereas SNP promoted all the studied parameters. Moreover, the generation of NO was also confirmed microscopically using a membrane permanent fluorochrome (4‐5‐diaminofluorescein diacetate [DAF‐2 DA]). Further, analysis showed that SMF enhanced the NR activity and triggered the NO production and NR was maximally decreased by ST as compared to LNAME, CPTIO and DPI. Thus, in addition to ROS, NO might be one of the important signaling molecules in magnetopriming‐induced salt tolerance in soybean and NR may be responsible for SMF‐triggered NO generation in roots of soybean.