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Responses of nitric oxide and hydrogen sulfide in regulating oxidative defence system in wheat plants grown under cadmium stress
Author(s) -
Kaya Cengiz,
Ashraf Muhammad,
Alyemeni Mohammed Nasser,
Ahmad Parvaiz
Publication year - 2020
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.13012
Subject(s) - sodium hydrosulfide , chemistry , cadmium , malondialdehyde , sodium nitroprusside , hydrogen peroxide , nitric oxide , proline , antioxidant , chlorophyll , food science , oxidative stress , catalase , dry matter , chlorophyll b , hydrogen sulfide , biochemistry , horticulture , botany , carotenoid , biology , organic chemistry , sulfur , amino acid
We conducted a study to evaluate the interactive effect of NO and H 2 S on the cadmium (Cd) tolerance of wheat. Cadmium stress considerably reduced total dry weight, chlorophyll a and b content and ratio of Fv/Fm by 36.7, 48.6, 26.7 and 19.5%, respectively, but significantly enhanced the levels of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA), endogenous H 2 S and NO, and the activities of antioxidant enzymes. Exogenously applied sodium nitroprusside (SNP) and sodium hydrosulfide (NaHS), donors of NO and H 2 S, respectively, enhanced total plant dry matter by 47.8 and 39.1%, chlorophyll a by 92.3 and 61.5%, chlorophyll b content by 29.1 and 27.2%, Fv/Fm ratio by 19.7 and 15.2%, respectively, and the activities of antioxidant enzymes, but lowered oxidative stress and proline content in Cd‐stressed wheat plants. NaHS and SNP also considerably limited both the uptake and translocation of Cd, thereby improving the levels of some key mineral nutrients in the plants. Enhanced levels of NO and H 2 S induced by NaHS were reversed by hypotuarine application, but they were substantially reduced almost to 50% by cPTIO (a NO scavenger) application. Hypotuarine was not effective, but cPTIO was highly effective in reducing the levels of NO and H 2 S produced by SNP in the roots of Cd‐stressed plants. The results showed that interactive effect of NO and H 2 S can considerably improve plant resistance against Cd toxicity by reducing oxidative stress and uptake of Cd in plants as well as by enhancing antioxidative defence system and uptake of some essential mineral nutrients.