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Structural isomers of the S 2 state in photosystem II: do they exist at room temperature and are they important for function?
Author(s) -
Chatterjee Ruchira,
Lassalle Louise,
Gul Sheraz,
Fuller Franklin D.,
Young Iris D.,
Ibrahim Mohamed,
de Lichtenberg Casper,
Cheah Mun Hon,
Zouni Athina,
Messinger Johannes,
Yachandra Vittal K.,
Kern Jan,
Yano Junko
Publication year - 2019
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12947
Subject(s) - electron paramagnetic resonance , photosystem ii , chemistry , oxygen evolving complex , crystallography , catalytic cycle , x ray absorption spectroscopy , photochemistry , molecule , photosystem i , photosynthetic reaction centre , absorption spectroscopy , catalysis , electron transfer , photosynthesis , nuclear magnetic resonance , biochemistry , physics , organic chemistry , quantum mechanics
In nature, an oxo‐bridged Mn 4 CaO 5 cluster embedded in photosystem II (PSII), a membrane‐bound multi‐subunit pigment protein complex, catalyzes the water oxidation reaction that is driven by light‐induced charge separations in the reaction center of PSII. The Mn 4 CaO 5 cluster accumulates four oxidizing equivalents to enable the four‐electron four‐proton catalysis of two water molecules to one dioxygen molecule and cycles through five intermediate S‐states, S 0 – S 4 in the Kok cycle. One important question related to the catalytic mechanism of the oxygen‐evolving complex (OEC) that remains is, whether structural isomers are present in some of the intermediate S‐states and if such equilibria are essential for the mechanism of the O‐O bond formation. Here we compare results from electron paramagnetic resonance (EPR) and X‐ray absorption spectroscopy (XAS) obtained at cryogenic temperatures for the S 2 state of PSII with structural data collected of the S 1 , S 2 and S 3 states by serial crystallography at neutral pH (∼6.5) using an X‐ray free electron laser at room temperature. While the cryogenic data show the presence of at least two structural forms of the S 2 state, the room temperature crystallography data can be well‐described by just one S 2 structure. We discuss the deviating results and outline experimental strategies for clarifying this mechanistically important question.