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Isolation and characterization of CAC antenna proteins and photosystem I supercomplex from the cryptophytic alga Rhodomonas salina
Author(s) -
Kuthanová Trsková Eliška,
Bína David,
Santabarbara Stefano,
Sobotka Roman,
Kaňa Radek,
Belgio Erica
Publication year - 2019
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12928
Subject(s) - photosystem ii , quenching (fluorescence) , phaeodactylum tricornutum , xanthophyll , dunaliella salina , photosynthesis , non photochemical quenching , fluorescence spectroscopy , biology , chemistry , photochemistry , fluorescence , algae , botany , physics , quantum mechanics
In the present paper, we report an improved method combining sucrose density gradient with ion‐exchange chromatography for the isolation of pure chlorophyll a/c antenna proteins from the model cryptophytic alga Rhodomonas salina . Antennas were used for in vitro quenching experiments in the absence of xanthophylls, showing that protein aggregation is a plausible mechanism behind non‐photochemical quenching in R. salina . From sucrose gradient, it was also possible to purify a functional photosystem I supercomplex, which was in turn characterized by steady‐state and time‐resolved fluorescence spectroscopy. R. salina photosystem I showed a remarkably fast photochemical trapping rate, similar to what recently reported for other red clade algae such as Chromera velia and Phaeodactylum tricornutum . The method reported therefore may also be suitable for other still partially unexplored algae, such as cryptophytes.