Premium
Ancient rice cultivar extensively replaces phospholipids with non‐phosphorus glycolipid under phosphorus deficiency
Author(s) -
Tawaraya Keitaro,
Honda Soichiro,
Cheng Weiguo,
Chuba Masaru,
Okazaki Yozo,
Saito Kazuki,
Oikawa Akira,
Maruyama Hayato,
Wasaki Jun,
Wagatsuma Tadao
Publication year - 2018
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12699
Subject(s) - phospholipid , glycolipid , cultivar , phosphatidylcholine , chemistry , phosphatidylinositol , phosphatidylglycerol , lysophosphatidylcholine , lipidome , diacylglycerol kinase , phosphorus , phosphatidylethanolamine , biochemistry , botany , galactolipid , food science , biology , lipid metabolism , enzyme , protein kinase c , organic chemistry , kinase , membrane
Recycling of phosphorus (P) from P‐containing metabolites is an adaptive strategy of plants to overcome soil P deficiency. This study was aimed at demonstrating differences in lipid remodelling between low‐P‐tolerant and ‐sensitive rice cultivars using lipidome profiling. The rice cultivars Akamai (low‐P‐tolerant) and Koshihikari (low‐P‐sensitive) were grown in a culture solution with [2 mg l −1 (+P)] or without (−P) phosphate for 21 and 28 days after transplantation. Upper and lower leaves were collected. Lipids were extracted from the leaves and their composition was analysed by liquid chromatography/mass spectrometry (LC–MS). Phospholipids, namely phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and phosphatidylinositol (PI), lysophosphatidylcholine (lysoPC), diacylglycerol (DAG), triacylglycerol (TAG) and glycolipids, namely sulfoquinovosyl diacylglycerol (SQDG), digalactosyldiacylglycerol (DGDG), monogalactosyldiacylglycerol (MGDG) and 1,2‐diacyl‐3‐O‐alpha‐glucuronosyl glycerol (GlcADG), were detected. GlcADG level was higher in both cultivars grown in −P than in +P and the increase was larger in Akamai than in Koshihikari. DGDG, MGDG and SQDG levels were higher in Akamai grown in −P than in +P and the increase was larger in the upper leaves than in the lower leaves. PC, PE, PG and PI levels were lower in both cultivars grown in −P than in +P and the decrease was larger in the lower leaves than in the upper leaves and in Akamai than in Koshihikari. Akamai catabolised more phospholipids in older leaves and synthesised glycolipids in younger leaves. These results suggested that extensive phospholipid replacement with non‐phosphorus glycolipids is a mechanism underlying low‐P‐tolerance in rice cultivars.