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Comparative proteomics illustrates the molecular mechanism of potato ( Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro
Author(s) -
Cheng Lixiang,
Wang Yuping,
Liu Yueshan,
Zhang Qingquan,
Gao Huihui,
Zhang Feng
Publication year - 2018
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12670
Subject(s) - proteome , proteomics , gibberellin , signal transduction , biology , biochemistry , microbiology and biotechnology , metabolic pathway , solanum tuberosum , transcription factor , metabolism , botany , gene
Among the multiple environmental signals and hormonal factors regulating potato tuberization, gibberellins (GAs) are important components of the signaling pathways in these processes. To understand the GAs‐signaling response mechanism of potato tuberization, a comparative proteomics approach was applied to analyze proteome change of potato tuberization in vitro subjected to a range of exogenous GA 3 treatments (0, 0.01, 0.1 and 1.0 μ M ) using two‐dimensional gel electrophoresis. Quantitative image analyses showed that a total of 37 protein spots have their abundance significantly altered more than 2‐fold. Among these proteins, 13 proteins were up‐regulated, 13 proteins were down‐regulated, one protein was absent and 10 proteins were induced after treatment by exogenous GA 3 . The MALDI‐TOF/TOF MS analyses led to the identification of differentially abundant proteins that are mainly involved in bioenergy and metabolism, storage, signaling, cell defense and rescue, transcription, chaperones, transport. Furthermore, the comparative analysis of GA 3 ‐responsive proteome allowed for general elucidation of underlying molecular mechanisms of potato tuberization inhibited by exogenous GA 3 . Most of these cellular processes were not conducive to the transition from stolon elongation to tuber formation, including a blockage of starch and storage protein accumulation, the accelerated carbohydrate catabolism, a blockage of JA biosynthesis but an elevated endogenous GAs level, the amplification of GA 3 signal transduction by other signaling pathways, and the regulation of cellular RNA metabolism for controlling tuberization. Our results firstly integrated physiology and proteome data to provide new insights into GA 3 ‐signaling response mechanisms of potato tuberization in vitro.