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Prominent alterations of wild barley leaf transcriptome in response to individual and combined drought acclimation and heat shock conditions
Author(s) -
Ashoub Ahmed,
Müller Niels,
JiménezGómez José M.,
Brüggemann Wolfgang
Publication year - 2018
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12667
Subject(s) - acclimatization , transcriptome , biology , gene , hordeum vulgare , hordeum , adaptation (eye) , heat shock protein , shock (circulatory) , heat shock , drought stress , rna seq , botany , poaceae , gene expression , genetics , medicine , neuroscience
Under field conditions, drought and heat stress typically happen simultaneously and their negative impact on the agricultural production is expected to increase worldwide under the climate change scenario. In this study, we performed RNA‐sequencing analysis on leaves of wild barley ( Hordeum spontaneum ) originated from the northern coastal region of Egypt following individual drought acclimation (DA) and heat shock (HS) treatments and their combination (CS, combined stresses) to distinguish the unique and shared differentially expressed genes (DEG). Results indicated that the number of unique genes that were differentially expressed following HS treatment exceeded the number of those expressed following DA. In addition, the number of genes that were uniquely differentially expressed in response to CS treatment exceeded the number of those of shared responses to individual DA and HS treatments. These results indicate a better adaptation of the Mediterranean wild barley to drought conditions when compared with heat stress. It also manifests that the wild barley response to CS tends to be unique rather than common. Annotation of DEG showed that metabolic processes were the most influenced biological function in response to the applied stresses.