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Hydrogen peroxide produced by NADPH oxidase: a novel downstream signaling pathway in melatonin‐induced stress tolerance in Solanum lycopersicum
Author(s) -
Gong Biao,
Yan Yanyan,
Wen Dan,
Shi Qinghua
Publication year - 2017
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12581
Subject(s) - melatonin , signal transduction , hydrogen peroxide , nadph oxidase , solanum , apx , abiotic stress , microbiology and biotechnology , biology , chemistry , oxidative stress , biochemistry , botany , gene , catalase , endocrinology
The beneficial effects of melatonin on abiotic stress have been demonstrated in several plants. However, little is known about the signal transduction pathway of melatonin involved in the plant stress response. Here, we manipulated the melatonin levels in tomato plants through a chemical approach. The roles of melatonin in stress tolerance were studied by assessing the symptoms, chlorophyll fluorescence and stress‐responsive gene expression. Moreover, both chemical and genetic approaches were used to study the roles of hydrogen peroxide ( H 2 O 2 ) in melatonin‐induced signal transduction in tomato plants. We found that melatonin activates NADPH oxidase ( RBOH ) to enhance H 2 O 2 levels by reducing its S ‐nitrosylation activity. Furthermore, melatonin‐induced H 2 O 2 accumulation was accompanied by obtainable stress tolerance. Inhibition of RBOH or chemical scavenging of H 2 O 2 significantly reduced the melatonin‐induced defense response, including reduced expression of several stress‐related genes ( CDPK1 , MAPK1 , TSPMS , ERF4 , HSP80 and ERD15 ) and reduced antioxidative enzyme activity ( SOD , CAT and APX ), which were responsible for the stress tolerance. Collectively, these results revealed a novel mechanism in which RBOH activity and H 2 O 2 signaling are important components of the melatonin‐induced stress tolerance in tomato plants.